rabbit polyclonal antibodies against ki67  (Thermo Fisher)

Journal: Nature Communications

Article Title: Dimeric copper peptide incorporated hydrogel for promoting diabetic wound healing

doi: 10.1038/s41467-025-61141-1

Figure Lengend Snippet: a Representative fluorescence images of Ki67 staining of HUVEC cells after different treatments (green: Ki67; blue: cell nucleus, Scale bars = 20 µm). b Quantitative analysis of Ki67+ cells. Data represented as Mean ± SD (n = 3). Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. c Relative cell viability of NIH/3T3 cells co-cultured with D-CuP solution and M-CuP solution for 24 h. Data represented as Mean ± SD (n = 4 independent replicates, ns not significant). Statistical significance was assessed using two-way ANOVA with Tukey’s multiple comparisons test. d Representative images of tube formation assay in HUVEC cells stained with calcein-AM (green) (Tube: yellow arrow, Scale bars = 400 µm). e Quantitative analysis of total tube length. Data represented as Mean ± SD (n = 3). Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. f The concentrations of VEGF were determined by ELISA. Data represented as Mean ± SD (n = 3 independent replicates). Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. g Representative images of transwell migration assay of L929 cells (Scale bars = 200 µm). h Quantitative analysis of total tube length. Data represented as Mean ± SD (n = 3). Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. i Images of L929 cells scratch experiment after incubating with D-CuP or M-CuP at different times (Scale bars = 100 μm). j Cell scratch healing rates at different times. Data represented as Mean ± SD (n = 3 independent replicates). Statistical significance was assessed using two-way ANOVA with Tukey’s multiple comparisons test. Source data are provided as a Source Data file.

Article Snippet: Cells were incubated with rabbit polyclonal antibody against Ki67 (1:500, Cat No. 28074-1-AP, Lot No. 00141310, Proteintech, China), followed by CoraLite488 goat anti-Rabbit IgG(H + L) secondary antibody (1:500, Cat No. SA00013-2, Lot No. 20000839, Proteintech, China).

Techniques: Fluorescence, Staining, Cell Culture, Tube Formation Assay, Enzyme-linked Immunosorbent Assay, Transwell Migration Assay

Journal: Nature Communications

Article Title: Dimeric copper peptide incorporated hydrogel for promoting diabetic wound healing

doi: 10.1038/s41467-025-61141-1

Figure Lengend Snippet: The concentrations of ( a ) IL-6, ( b ) IL-10, and d VEGF in the wound tissue were determined by ELISA on day 3. Data represented as Mean ± SD. (n = 3 independent replicates) Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. c Representative images of immunohistochemistry staining of TNF-α, VEGF, CD31, and Ki67 (Scale bars = 100 μm) on day 7 and Masson staining on day 12 (Scale bars = 500 μm). Quantitative analysis of immunohistochemical staining for ( e ) CD31 and ( f ) Ki67 on day 7. Data represented as Mean ± SD. (n = 3 independent replicates) Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. g Quantification of the collagen deposition in different groups on day 12. Data represented as Mean ± SD (n = 3 independent replicates). Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. Source data are provided as a Source Data file.

Article Snippet: Cells were incubated with rabbit polyclonal antibody against Ki67 (1:500, Cat No. 28074-1-AP, Lot No. 00141310, Proteintech, China), followed by CoraLite488 goat anti-Rabbit IgG(H + L) secondary antibody (1:500, Cat No. SA00013-2, Lot No. 20000839, Proteintech, China).

Techniques: Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Staining, Immunohistochemical staining

Journal: Cancers

Article Title: Melatonin Modulates the SIRT1-Related Pathways via Transdermal Cryopass-Laser Administration in Prostate Tumor Xenograft

doi: 10.3390/cancers15204908

Figure Lengend Snippet: Representative xenograft photomicrographs of hematoxylin-eosin ( A , B ) and Sirius red ( C , D ), polarized Sirius red ( E , F ) morphological stainings and of Ki67 expression ( G , H ) on vehicle cryopass-laser-treated mice ( A , C , E , G ) and melatonin cryopass-laser-treated mice ( B , D , F , H ). Bars: 8 μm. The green asterisks indicate the extravascular red blood cells, the yellow arrows indicate the tumor cells nuclei with irregular shape, the blue arrows indicate collagen fibers, and the red arrows indicate Ki67 positivity. Graph ( I ) summarizes the number of Ki67 positive cells/field measurement. * p ≤ 0.05 vs. melatonin cryopass-laser group.

Article Snippet: The xenograft sections were incubated for 1 h at 37 °C and for 1 h at room temperature with the following primary antibodies: rabbit polyclonal antibody against anti-Ki67 (diluted 1:100; Abcam, Cambridge, UK); anti-CD31; goat polyclonal antibody against MMP2 (diluted 1:200; Santa Cruz Biotechnology, Dallas, TX, USA); rabbit polyclonal antibody against SIRT1 (diluted 1:250; Abcam, Cambridge, UK); rabbit polyclonal antibody against PGC-1α (diluted 1:400; Abcam, Cambridge, UK); rabbit polyclonal PPAR γ (diluted 1:500; Abcam, Cambridge, UK; and rabbit polyclonal against NF-kB (diluted 1:350; Abcam, Cambridge, UK).

Techniques: Expressing